Comparative Analysis of Whole Transcriptome Profiles in Septic Cardiomyopathy: Insights from CLP- and LPS-Induced Mouse Models

Sepsis is a life-threatening organ dysfunction caused by dysregulated host response to infection, with septic cardiomyopathy being common and severe complication. Despite its significant clinical impact, the molecular mechanisms underlying sepsis-induced (SICM) remain incompletely understood. In this study, we performed comparative analysis of whole transcriptome profiles using RNA sequencing in mouse hearts two widely used models cardiomyopathy. CLP-induced sepsis was achieved surgical cecal ligation puncture, while LPS-induced induced 5 mg/kg intraperitoneal (IP) injection lipopolysaccharide (LPS). For consistency, utilized sham-operated mice as control for models. Our aim identify key genes pathways involved development evaluate similarities differences between findings demonstrated that both CLP LPS methods could induce heart within 24 h. We identified transcriptional regulatory regions models, such Nfkb1, Sp1, Jun. Moreover, differentially expressed (DEGs) comparison were shared pathways, including regulation inflammatory response, reactive oxygen species metabolic process, JAK-STAT signaling pathway. However, each model presented distinctive expression potentially diverse contributing failure. This extensive enhances our understanding basis cardiomyopathy, providing invaluable insights. Accordingly, study also contributes pursuit effective personalized treatment strategies SICM, highlighting importance considering specific causative factors.